Regulatory

Part:BBa_K4207011:Design

Designed by: Jesper Mickos   Group: iGEM22_Aboa   (2022-09-30)


BYDV toehold switch B39 by TrigGate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This toehold switch was designed according to the B-series toehold structure (Pardeee et. al., 2016). The A-series structure was designed to reduce translational leakage compared to older toehold switches (Green et. al., 2014). The binding site of the toehold switch was designed to bind to a conserved sequence in the BYDV genome, allowing it to sense the presence of this trigger sequence. This toehold switch consists of a 30-nt linker sequence from sensor 27B (Pardee et. al. (2016)) upstream of the Type IIS junction site as opposed to the more conventional 21-nt linker sequence of toehold switches. The last nucleotide of this linker sequence is part of the junction site (AATG), so it is not registered here.


Source

This part was created in collaboration with TrigGate, using 10 different barley yellow dwarf virus (BYDV) whole genomes to screen for conserved sequences to be detected by the toehold switches.

References